INVESTIGADORES
MORGAN Patricio Eduardo
congresos y reuniones científicas
Título:
Effect of cardiac EGFR silencing with siRNA on the slow force response to myocardial stretch
Autor/es:
BREA MS, DÍAZ RG, ESCUDERO DS, LEPORACE GUIMIL J, CALDIZ CI, PÉREZ NG, MORGAN PE
Lugar:
Rosario
Reunión:
Congreso; L Reunión de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB) 2014; 2014
Resumen:
The stretch of cardiac
muscle produces an initial fast increase in force, followed by a slow force
response (SFR). The SFR seems to be due to an autocrine mechanism that involves
the activation
of the epidermal growth factor receptor (EGFR) and the consequent generation
and release of reactive oxygen species (ROS). We developed a lentivirus
carrying shEGFR sequence to inhibit EGFR expression, without affecting cardiac
family members ErbB2 and ErbB4, and injected into a rat cardiac left ventricular
wall (n=5). As control, a second group of sham rats were injected with
phosphate buffer saline (PBS, n=5). Four-week later, EGFR protein expression in
the left ventricle of shEGFR expressing rats showed 52 ± 15 % reduction compared to sham animals (100 ±
6 %, P<0.05). The SFR, evaluated in isolated left ventricle papillary muscles,
was blunted in animals expressing shEGFR (in % of initial phase: 102 ± 1 vs. sham
112 ± 1, P<0.05). ROS production (evaluated by the lucigenin method) was
assessed in cardiac left ventricle stripes after AII (1 nM) and EGF (0.1 mg/ml) stimulation. AII and EGF increased basal ROS production
in sham rats by 47 ± 2 and 43 ± 6 % respectively, effect that was significantly
reduced in shEGFR expressing rats (5 ± 5 and 17 ± 12 % respectively). We
conclude that EGFR activation is crucial for the redox-sensitive mechanism that
triggers the SFR to myocardial stretch.