INVESTIGADORES
AGUSTI Rosalia
artículos
Título:
The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids.
Autor/es:
AGUSTI, R.; COUTO, A.S.; CAMPETELLA, O.; FRASCH, A.C.C.; LEDERKREMER, R.M.
Revista:
GLYCOBIOLOGY
Editorial:
Oxford University Press
Referencias:
Lugar: Oxford; Año: 1997 vol. 7 p. 731 - 735
ISSN:
0959-6658
Resumen:
The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-hexadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. cruzi was made.
