Interferon-γ and IL-10 Release Assay for patients with ocular toxoplasmosis

REINA SILVIA; RUDZINSKI, MARCELO; PARDINI, LAIS; BERSTEIN, MARIANA; MORE, GASTON

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

AMER SOC TROP MED & HYGIENE

Lugar: Stanford; Año: 2020

0002-9637

Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with Toxoplasma gondii total lysate antigen (TLA) in a cytokine releasing assay (CRA), run during the inactive period of the disease. IFN-γ and IL-10 levels were measured. Increased IFN-γ levels were detected after PBMC stimulation with either ME49 reference strain (p=0.0015) or local TgCkAr 11-9 isolate (p=0.0012) reference strains, as compared with those recorded under basal conditions. TLA from TgCkAr11-9 isolatestrain induced a higher release of IFN-γ than ME49 in CRA from all tested patients (p=0.02). The median value of IFN-γ release upon TgCkAr 11-9 stimulation (26.03pg/ml) allowed the classification of patients into high- or low- / non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains or isolates when performing CRA with T. gondii TLA.