Action of Anti-M3 muscarinic acetylcholine receptor IgG of primary Sjögren`s syndrome on the enzymatic antioxidant system in rat submandibular gland

REINA, SILVIA; MARCELO RODRIGUEZ; STRANIERI, GRACIELA; BORDA, ENRI

JOURNAL OF ORAL PATHOLOGY AND MEDICINE

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2015 vol. 1 p. 1 - 8

0904-2512

BACKGROUND: We demonstrate that serum immonuglobulin G (IgG) directed against glandular M3 muscarinic acethylcholine receptors (M3 mAChR) and pilocarpine triggers the increment of superooxidase dismutase (SOD) and catalase (CAT) and the production of nitric oxide (NO) and prostaglandin E2 (PGE2). METHODS: Enzyme-linked immunoabsorbent assay (ELISA) was performed in the presence of the human M3 mAChR synthetic peptide as antigen to detect in serum of pSS patients the autoantibodies. Further, SOD and CAT specific activity and NO were determined chemically in the presence of anti-M3 mAChR IgG and pilocarpine. The level of PGE2 generation in the presence of autoantibody and pilocarpine was determined by ELISA. RESULTS: An association between anti-M3 mAChR autoantibodies and pilocarpine given the increment of the specific activity of SOD and CAT in the serum of pSS patients and in the rat submandibular gland was observed. As a result of this action, M3 synthetic peptide and atropine abrogated the stimulatory action. The L-type calcium channel, calcium/calmodulin complex and COX-2 inhibitors selectively blocked the increment of the specific activity of SOD and CAT in the rat submandibular gland. An increased production of NO and PGE2 by the cholinergic autoantibody and pilocarpine was also been detected. CONCLUSION: On the basis of these results, the increment of the specific activity of SOD and CAT in pSS patients as compared to control healthy individuals may be seen as a defensive reaction to the increment of the amount of ROS in the body, which becoming uncontrollable, leads to irreversible cellular and tissue damage.