INVESTIGADORES
PARREÑO Gladys Viviana
artículos
Título:
Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina.
Autor/es:
MAIDANA, SILVINA; LOMONACO, MARINA; COMBESSIES, G.; CRAIG, MARIA ISABEL; PARREÑO, VIVIANA; THIRY, ETIENE; ROMERA, ALEJANDRA
Revista:
BMC VETERINARY RESEARCH
Editorial:
BIOMED CENTRAL LTD
Referencias:
Lugar: Londres; Año: 2012 vol. 8 p. 1 - 9
ISSN:
1746-6148
Resumen:
Abstract
Background: Parainfluenza virus type 3 (PIV3) was isolated from dairy buffaloes (Bubalus bubalis) naturally affected
with respiratory and reproductive clinical conditions.
with respiratory and reproductive clinical conditions.
Parainfluenza virus type 3 (PIV3) was isolated from dairy buffaloes (Bubalus bubalis) naturally affected
with respiratory and reproductive clinical conditions.
Results: Examination of nasal and vaginal swabs collected from 12 diseased buffaloes led to the isolation of three
paramyxovirus isolates from two animals. Antigenic, morphological and biological characteristics of these three
isolates were essentially similar to those of members of the Paramyxoviridae family. Antigenic analysis by direct
immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus
type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo
isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to
genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).
immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus
type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo
isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to
genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).
paramyxovirus isolates from two animals. Antigenic, morphological and biological characteristics of these three
isolates were essentially similar to those of members of the Paramyxoviridae family. Antigenic analysis by direct
immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus
type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo
isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to
genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).
immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus
type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo
isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to
genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).
Examination of nasal and vaginal swabs collected from 12 diseased buffaloes led to the isolation of three
paramyxovirus isolates from two animals. Antigenic, morphological and biological characteristics of these three
isolates were essentially similar to those of members of the Paramyxoviridae family. Antigenic analysis by direct
immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus
type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo
isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to
genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).
immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus
type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo
isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to
genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).
Paramyxoviridae family. Antigenic analysis by direct
immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus
type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo
isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to
genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).
Conclusions: This is the first characterization of BPIV3 in water buffalo.
According to the samples analyzed, in Argentina, the genotype B was found in buffalo and the genotypes A and C
were found in cattle.
According to the samples analyzed, in Argentina, the genotype B was found in buffalo and the genotypes A and C
were found in cattle.
This is the first characterization of BPIV3 in water buffalo.
According to the samples analyzed, in Argentina, the genotype B was found in buffalo and the genotypes A and C
were found in cattle.