INVESTIGADORES
PARREÑO Gladys Viviana
artículos
Título:
Molecular Characterization of Equine Rotaviruses Circulating in Argentinean Foals during a 17-Year Surveillance Period (1992-2008).
Autor/es:
GARAICOECHEA, LORENA; MIÑO, SAMUEL; CIARLET, MAX; FERNANDEZ, FERNANDO; BARRANDEGUY, MARIA; PARREÑO, VIVIANA
Revista:
VETERINARY MICROBIOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2011 vol. 24 p. 150 - 160
ISSN:
0378-1135
Resumen:
P[12]G3 and P[12]G14 equine rotaviruses (ERVs) are epidemiologically important in
horses. In Argentina, the prevalent ERV strains have been historically P[12]G3. The aim of
this study was the detection and characterization of ERV strains circulating in foals in
Argentina during a 17-year study (19922008). Additionally, the gene sequences of VP7,
VP4 and NSP4 encoding genes of representative Argentinean ERV strainswere determined
and phylogenetic analyses were performed to elucidate the evolutionary relationships of
the ERV strains in Argentina. ERVs were detected in 165 (21%) out of 771 diarrheic stool
samples, which corresponded to 45 (39%) of 116 outbreaks from the surveyed
thoroughbred horse farms. From the positive cases, 51% (n = 23) were G3, 33% (n = 15)
were G14, 4% (n = 2) represented a G3 + G14 mixed infection and 11% (n = 5) of the cases
could not be characterized. G3 ERV was detected during the entire period, while G14 ERV
was first detected in 2000 and increased its incidence specially in 2006 and 2007. All the
analyzed strains belonged to the VP4 P[12] genotype, except for one G3 case which
belonged to the P[3] genotype, constituting the first report of a P[3]G3 ERV strain.
Phylogenetic analysis of VP7 protein revealed that the G3 Argentinean ERV strains
clustered with ERVs from Ireland, while the G14 Argentinean ERV strains formed a distinct
cluster within the G14 genotype. The VP4 of the P[12] ERV strains clustered with P[12]
strains from Ireland and France. The NSP4 of the Argentinean ERV strains clustered with
the NSP4 genotype E12, along with those of guanaco and bovine strains from Argentina,
suggesting the a close evolutionary relationship among these Argentinean strains. The
results of this study showed changes in the incidence of G3 and G14 during the studied
period. The increase in the frequency of G14 ERV, not included in the vaccine, in the second
half of the period, may have implications for vaccine design.
could not be characterized. G3 ERV was detected during the entire period, while G14 ERV
was first detected in 2000 and increased its incidence specially in 2006 and 2007. All the
analyzed strains belonged to the VP4 P[12] genotype, except for one G3 case which
belonged to the P[3] genotype, constituting the first report of a P[3]G3 ERV strain.
Phylogenetic analysis of VP7 protein revealed that the G3 Argentinean ERV strains
clustered with ERVs from Ireland, while the G14 Argentinean ERV strains formed a distinct
cluster within the G14 genotype. The VP4 of the P[12] ERV strains clustered with P[12]
strains from Ireland and France. The NSP4 of the Argentinean ERV strains clustered with
the NSP4 genotype E12, along with those of guanaco and bovine strains from Argentina,
suggesting the a close evolutionary relationship among these Argentinean strains. The
results of this study showed changes in the incidence of G3 and G14 during the studied
period. The increase in the frequency of G14 ERV, not included in the vaccine, in the second
half of the period, may have implications for vaccine design.
were G14, 4% (n = 2) represented a G3 + G14 mixed infection and 11% (n = 5) of the cases
could not be characterized. G3 ERV was detected during the entire period, while G14 ERV
was first detected in 2000 and increased its incidence specially in 2006 and 2007. All the
analyzed strains belonged to the VP4 P[12] genotype, except for one G3 case which
belonged to the P[3] genotype, constituting the first report of a P[3]G3 ERV strain.
Phylogenetic analysis of VP7 protein revealed that the G3 Argentinean ERV strains
clustered with ERVs from Ireland, while the G14 Argentinean ERV strains formed a distinct
cluster within the G14 genotype. The VP4 of the P[12] ERV strains clustered with P[12]
strains from Ireland and France. The NSP4 of the Argentinean ERV strains clustered with
the NSP4 genotype E12, along with those of guanaco and bovine strains from Argentina,
suggesting the a close evolutionary relationship among these Argentinean strains. The
results of this study showed changes in the incidence of G3 and G14 during the studied
period. The increase in the frequency of G14 ERV, not included in the vaccine, in the second
half of the period, may have implications for vaccine design.
could not be characterized. G3 ERV was detected during the entire period, while G14 ERV
was first detected in 2000 and increased its incidence specially in 2006 and 2007. All the
analyzed strains belonged to the VP4 P[12] genotype, except for one G3 case which
belonged to the P[3] genotype, constituting the first report of a P[3]G3 ERV strain.
Phylogenetic analysis of VP7 protein revealed that the G3 Argentinean ERV strains
clustered with ERVs from Ireland, while the G14 Argentinean ERV strains formed a distinct
cluster within the G14 genotype. The VP4 of the P[12] ERV strains clustered with P[12]
strains from Ireland and France. The NSP4 of the Argentinean ERV strains clustered with
the NSP4 genotype E12, along with those of guanaco and bovine strains from Argentina,
suggesting the a close evolutionary relationship among these Argentinean strains. The
results of this study showed changes in the incidence of G3 and G14 during the studied
period. The increase in the frequency of G14 ERV, not included in the vaccine, in the second
half of the period, may have implications for vaccine design.
n = 23) were G3, 33% (n = 15)
were G14, 4% (n = 2) represented a G3 + G14 mixed infection and 11% (n = 5) of the cases
could not be characterized. G3 ERV was detected during the entire period, while G14 ERV
was first detected in 2000 and increased its incidence specially in 2006 and 2007. All the
analyzed strains belonged to the VP4 P[12] genotype, except for one G3 case which
belonged to the P[3] genotype, constituting the first report of a P[3]G3 ERV strain.
Phylogenetic analysis of VP7 protein revealed that the G3 Argentinean ERV strains
clustered with ERVs from Ireland, while the G14 Argentinean ERV strains formed a distinct
cluster within the G14 genotype. The VP4 of the P[12] ERV strains clustered with P[12]
strains from Ireland and France. The NSP4 of the Argentinean ERV strains clustered with
the NSP4 genotype E12, along with those of guanaco and bovine strains from Argentina,
suggesting the a close evolutionary relationship among these Argentinean strains. The
results of this study showed changes in the incidence of G3 and G14 during the studied
period. The increase in the frequency of G14 ERV, not included in the vaccine, in the second
half of the period, may have implications for vaccine design.
could not be characterized. G3 ERV was detected during the entire period, while G14 ERV
was first detected in 2000 and increased its incidence specially in 2006 and 2007. All the
analyzed strains belonged to the VP4 P[12] genotype, except for one G3 case which
belonged to the P[3] genotype, constituting the first report of a P[3]G3 ERV strain.
Phylogenetic analysis of VP7 protein revealed that the G3 Argentinean ERV strains
clustered with ERVs from Ireland, while the G14 Argentinean ERV strains formed a distinct
cluster within the G14 genotype. The VP4 of the P[12] ERV strains clustered with P[12]
strains from Ireland and France. The NSP4 of the Argentinean ERV strains clustered with
the NSP4 genotype E12, along with those of guanaco and bovine strains from Argentina,
suggesting the a close evolutionary relationship among these Argentinean strains. The
results of this study showed changes in the incidence of G3 and G14 during the studied
period. The increase in the frequency of G14 ERV, not included in the vaccine, in the second
half of the period, may have implications for vaccine design.
n = 2) represented a G3 + G14 mixed infection and 11% (n = 5) of the cases
could not be characterized. G3 ERV was detected during the entire period, while G14 ERV
was first detected in 2000 and increased its incidence specially in 2006 and 2007. All the
analyzed strains belonged to the VP4 P[12] genotype, except for one G3 case which
belonged to the P[3] genotype, constituting the first report of a P[3]G3 ERV strain.
Phylogenetic analysis of VP7 protein revealed that the G3 Argentinean ERV strains
clustered with ERVs from Ireland, while the G14 Argentinean ERV strains formed a distinct
cluster within the G14 genotype. The VP4 of the P[12] ERV strains clustered with P[12]
strains from Ireland and France. The NSP4 of the Argentinean ERV strains clustered with
the NSP4 genotype E12, along with those of guanaco and bovine strains from Argentina,
suggesting the a close evolutionary relationship among these Argentinean strains. The
results of this study showed changes in the incidence of G3 and G14 during the studied
period. The increase in the frequency of G14 ERV, not included in the vaccine, in the second
half of the period, may have implications for vaccine design.