INVESTIGADORES
PARREÑO Gladys Viviana
artículos
Título:
IgY Antibodies Protect Against Human Rotavirus Induced Diarrhea in the Neonatal Gnotobiotic Piglet Disease Model
Autor/es:
VEGA, CELINA; BOK, MARINA; VLASOVA, A.; CHATTHA, K; FERNANDEZ, FERNANDO; ANDRÉS WIGDOROVITZ; PARREÑO, VIVIANA; SAIF, LINDA J.
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2012 vol. 7 p. 1 - 16
ISSN:
1932-6203
Resumen:
Group A Rotaviruses are the most common cause of severe, dehydrating diarrhea in children worldwide. The aim of the
present work was to evaluate protection against rotavirus (RV) diarrhea conferred by the prophylactic administration of
specific IgY antibodies (Ab) to gnotobiotic piglets experimentally inoculated with virulent Wa G1P[8] human rotavirus (HRV).
Chicken egg yolk IgY Ab generated from Wa HRV hyperimmunized hens specifically recognized (ELISA) and neutralized Wa
HRV in vitro. Supplementation of the RV Ab free cow milk diet with Wa HRV-specific egg yolk IgY Ab at a final ELISA Ab titer
of 4096 (virus neutralization VN- titer = 256) for 9 days conferred full protection against Wa HRV associated diarrhea and
significantly reduced virus shedding. This protection was dose-dependent. The oral administration of semi-purified passive
IgY Abs from chickens did not affect the isotype profile of the pig Ab secreting cell (ASC) responses to Wa HRV infection, but
it was associated with significantly fewer numbers of HRVspecific IgA ASC in the duodenum. We further analyzed the pigs
immune responses to the passive IgY treatment. The oral administration of IgY Abs induced IgG Ab responses to chicken IgY
in serum and local IgA and IgG Ab responses to IgY in the intestinal contents of neonatal piglets in a dose dependent
manner. To our knowledge, this is the first study to show that IgY Abs administered orally as a milk supplement passively
protect neonatal pigs against an enteric viral pathogen (HRV). Piglets are an animal model with a gastrointestinal physiology
and an immune system that closely mimic human infants. This strategy can be scaled-up to inexpensively produce large
amounts of polyclonal IgY Abs from egg yolks to be applied as a preventive and therapeutic passive Ab treatment to
control RV diarrhea.in vitro. Supplementation of the RV Ab free cow milk diet with Wa HRV-specific egg yolk IgY Ab at a final ELISA Ab titer
of 4096 (virus neutralization VN- titer = 256) for 9 days conferred full protection against Wa HRV associated diarrhea and
significantly reduced virus shedding. This protection was dose-dependent. The oral administration of semi-purified passive
IgY Abs from chickens did not affect the isotype profile of the pig Ab secreting cell (ASC) responses to Wa HRV infection, but
it was associated with significantly fewer numbers of HRVspecific IgA ASC in the duodenum. We further analyzed the pigs
immune responses to the passive IgY treatment. The oral administration of IgY Abs induced IgG Ab responses to chicken IgY
in serum and local IgA and IgG Ab responses to IgY in the intestinal contents of neonatal piglets in a dose dependent
manner. To our knowledge, this is the first study to show that IgY Abs administered orally as a milk supplement passively
protect neonatal pigs against an enteric viral pathogen (HRV). Piglets are an animal model with a gastrointestinal physiology
and an immune system that closely mimic human infants. This strategy can be scaled-up to inexpensively produce large
amounts of polyclonal IgY Abs from egg yolks to be applied as a preventive and therapeutic passive Ab treatment to
control RV diarrhea.