Anti-inflammatory role of Galectin 8in Trypanosoma cruzi-induced myocarditis

PASCUALE CA; AOKI, MARIA P.; SANMARCO, LILIANA M.; POSTAN M; BERTELLI A; BENATAR A; LEGUIZAMON MS

Congreso; XXXV Reunião Anual da Sociedade Brasileira de Protozoologia XLVI Reunião Anual sobre Pesquisa básica em Doença de Chagas; 2019

Anti-inflammatory role of Galectin 8in Trypanosoma cruzi-induced myocarditis BERTELLI, A.1, SANMARCO, L.2, PASCUALE,C. 1, BENATAR, A3. POSTAN, M.4, AOKI, P.2 and LEGUIZAMÓN MS11 Instituto de InvestigacionesBiotecnológicas, UNSAM-CONICET 2 Centro de Investigación enBioquímica Clínica e Inmunología (CIBICI)-CONICET. Dpto de Bioquímica Clínica,Facultad de Ciencias Químicas, UNC. 3 Instituto de Investigaciones en IngenieríaGenética y Biología Molecular (INGEBI-CONICET), 4Instituto deParasitología M.Fatala Chabén  Galectinsconstitute a family of lectins that contain characteristic aminoacid sequencesand hold affinity for β-galactosides. Galectin-8 (Gal-8) is included in thetandem repeat group. It has been involved in cellular adhesion,migration, apoptosis, etc., is widely distributed in different tissues and can worksthrough an autocrine/paracrine way. In addition, different authors haveproposed Gal-8 to be involved either in pro-inflammatory or anti-inflammatory events.It is known that T. cruzi cardiomyopathyis based on the induction of a strong inflammation by the parasite persistence intissue. In order to analyse the role of Gal-8 in an inflammatory infectiouscontext, we used a murine chronic T.cruzi infection model: C57BL/6J (B6, WT) and B6Gal-8 knock out (KO) mice, infectedwith T. cruzi (Ac strain DTU TcI).Mice were sacrificed at 4 months post-infection. KO cardiac tissue showedincreased inflammation score in comparison with WT (p=0.0119). No differenceswere observed in fibrosis degree and tissue parasites level. In order toidentify the immune population in inflamed cardiac tissue, a flow-cytometryanalysis was performed. Similar values were found in the percentage of cardiacT lymphocytes (CD3+), and their different subpopulations, between KO and WTmice. We observed a rise in the frequency of macrophages (p=0.0021) in KO hearttissue compared to WT, in agreement with increased cardiac CCL-2 levels. Theincrement in neutrophil numbers observed in KO versus WT (p=0.0097) did notcorrelate with cardiac and systemic CXCL1 and CXCL2 levels. These resultssuggest that neutrophil rise may be instead related to Gal-8 preaparesisinduction mechanism.Takentogether, our results suggest that Gal-8 participate as an anti-inflammatory moleculein T. cruzi chronic infection.  <!-- /* Font Definitions */@font-face{font-family:Arial;panose-1:2 11 6 4 2 2 2 2 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536859905 -1073711037 9 0 511 0;}@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1073786111 1 0 415 0;} /* Style Definitions */p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin-top:0cm;margin-right:0cm;margin-bottom:8.0pt;margin-left:0cm;line-height:107%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:Calibri;mso-bidi-font-family:"Times New Roman";mso-ansi-language:ES-AR;mso-fareast-language:EN-US;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:10.0pt;mso-ansi-font-size:10.0pt;mso-bidi-font-size:10.0pt;font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;mso-hansi-font-family:Calibri;}size:595.3pt 841.9pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:35.4pt;mso-footer-margin:35.4pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}