Comparison between three score systems using HET-CAM method and in vivo eye irritation evaluation for cosmetics products in Argentina

RIVERO, MARTIN NICOLAS; PEREZ DAMONTE, SILVIA; WIKINSKI, SILVIA; GUTIÉRREZ, MARÍA LAURA

Río de Janeiro

Conferencia; 2nd PanAmerican Conference for Alternative Methods; 2018

CAAT - Center for Alternative to Animal Testing

The hens egg test on chorioallantoic membrane(HET-CAM) was used for in vitro evaluation of 10 cosmetic products (cream, micellar waters, serum and shampoo), controls (SDS 1%, 0.1 N NaOH and physiological solution) and Texapon ASV 5% as a benchmark. Fertilized hen?s eggs were used at 9 - 10 incubation day. The solutions were exposed 20 seconds, rinsed and the appearance of hyperemia/lysis, hemorrhage and coagulation was registered for 300 seconds. The responses were quantified according to the predictive models of Luepke (1985), and protocols n° 47 and n° 96 of INVITTOX-ECVAM. On the other hand, the in vivo response for the 10 cosmetics was clinically evaluated in human volunteers for 21 days. Symptoms as burning, itching, lacrimation, hyperemia, secretion and local tolerance were registered and the type of crystallization of the tear film was analyzed. The different in vitro classification systems were compared with the in vivo results.A greater correlation with in vivo results was found with Luepke and ECVAM protocol n° 47 score systems while the classification obtained with ECVAM protocol n° 96 did not show a good correlation. The 100% of the compounds identified as no irritant by HET-CAM were coincident with in vivo results. None false negatives were observed, showing therefore that HET-CAM is a good method to ensure that a compound does not produce irritation. The 20% of the compounds identified as irritants by the HET-CAM method have also been found irritants in vivo. The other 80% did not manifest symptoms of irritation in vivo, incurring in false positives. This could be due to the particular sensitivity of the CAM to some surfactants present in the cosmetics tested.This is the first assay comparing in vitro and in vivo methodologies in Argentina, an experience that may lay out the basis for the development and implementation of alternative methods to the use of laboratory animals in the evaluation of cosmetics.