IMPLEMENTATION OF ALTERNATIVE METHODS IN ARGENTINA TO EVALUATE SKIN AND OCULAR IRRITATION OF COSMETICS PRODUCTS

GERMÁN POLIZZI; MARIANO NIGRO; ESTEBAN PREUX; LEANDRO SUBIAGA; FIORELLA PANICHELLI; RODRIGO DE VECCHI; PABLO QUIROGA; GUTIÉRREZ, MARÍA LAURA

Niagara Falls

Congreso; 12th World Congress on Alternatives and Animal Use in the Life Sciences; 2023

Alternative Congress Trust

The implementation of alternative methods in Argentina is a new challenge. Althoughthere are currently no laws that restrict or prohibit the use of animals for the evaluationof cosmetic products in Argentina, due to ethical issues, social demand and to keep upwith global advances, it is necessary to work on the implementation of methodologieswithout animals.For this reason, Laboratorios Bagó SA decided to bet on the implementation of in vitrotests. It was decided to start with two tests to evaluate ocular irritation potential: STE(Short Time Exposure - OECD TG 491) and HET-CAM (hen's egg chorioallantoicmembrane test); and an assay to assess skin irritation: SkinEthic™ OECD Test Guideline439.In a first stage, proficiency tests were carried out with chemical products recommendedby the guidelines. Once methodological competence had been demonstrated, a series oftest were carried out. We evaluate 48 products by HET-CAM, 15 products by STE and 8products by SkinEthic. Among the product tested were antiseptic and healing creams;spray, cream and emulsion sunscreens; hydrating creams; line of hair products(shampoos and conditioners); facial and body creams; facial serum and raw materialsand ingredients.The implementation of the methodology was carried out successfully, no more animaltests are carried out for the evaluation of skin and eye irritation in Bagó. We arecurrently working on the implementation of an in vitro approach for the evaluation ofskin sensitization to definitively replace the use of animals in the evaluation of cosmeticproducts.