INVESTIGADORES
ROSSI Franco Ruben
congresos y reuniones científicas
Título:
EFFECT OF ARSENIC ON NADPH GENERATING ENZYMES IN SOYBEAN (GLYCINE MAX. L)
Autor/es:
ROSSI F,; MARSÁ S,; ZIRULNIK F.
Lugar:
Potrero de los Funes-San Luis
Reunión:
Congreso; CUYO BIOLOGY SOCIETY XXIV Annual Scientific Meeting; 2006
Institución organizadora:
Sociedad de Biologia de Cuyo
Resumen:
Species of inorganic arsenic produce oxidative stress, generating free
radicals and reactive oxygen species. These species react with lipids,
proteins, pigments and nucleic acids, producing lipoperoxidation,
enzymatic inactivation, so affecting the cellular viability. The role
of NADPH generating enzymes as NADP-isocitrate dehydrogenase
(ICD) in the antioxidative response of soybean leaves and
roots to arsenic contamination was studied. Grown plants were
exposed on the 10th day of adaptation to hydroponic conditions in
Hoaglands solution to intoxication with arsenic (6 and 16 uM)
during 24 and 72 hours. The enzymatic activity of ICD was determined
in soybean leaves and roots. The results showed a significant
increase at short time (24h) with treatment I (6uM) and at
long time (72h) with treatment II (16uM), in leaves (p < 0.001).
Roots showed an increase of the specific activity at 24h with treatment
I (p < 0.05).We can suggest that arsenic alters the NADPH
production, demonstrating an essential role of this cofactor to maintain
the reduction equivalents on the GSH-GSSG system, as part
of the antioxidative defense responses, depending on the arsenic
concentration and the time of treatment.