INVESTIGADORES
ROSSI Franco Ruben
congresos y reuniones científicas
Título:
OXIDATIVE STRESS AND ANTIOXIDANT DEFENSES IN Glycine max L. TREATED WITH ARSENIC
Autor/es:
MARSA S,; ROSSI F.; MOLINA A.; PEREZ CHACA MV.; ZIRULNIK F.
Lugar:
Tafí del Valle-Tucumán
Reunión:
Congreso; XXII Annual Scientific Meeting, Tucuman Biology Society. TUCUMAN - ARGENTINA.; 2005
Institución organizadora:
Sociedad de Biología de Tucumán
Resumen:
Introduction: Species of inorganic arsenic produce oxidative stress,
generating free radicals and reactive oxygen species. These species
react with lipids, proteins, pigments and nucleic acids, producing
lipoperoxidation, enzymatic inactivation, so affecting the
cellular viability. Objectives: a-Determine lipoperoxidaci.n parameters
like TBARS (Thiobarbituric acid reactive species). b- Measure
reduced Glutathione (GSH), non protein thiols (TNP) and
phytochelatins (FQ), in leaves and roots of Glycine max. L. Materials
and Methods: Roots and leaves with 24 and 72 h of intoxication
with arsenic (16 and 160 mM), in plants with 10 days of development,
using like control plants with equal development free of
arsenic. MDA was measured according to Heath (1968). GSH by
the method of Anderson (1985), TNP by Gallego, (1999) and FQ
were determined by difference between the TNP and GSH. Results:
TBARS showed a significant increase in leaves (p<0.001),
roots showed a significant decreased (p<0.01). TNP showed a similar
behavior respect to TBARS in leaves (p<0.01), in roots a decrease
was observed at 24 h (p<0.01). GSH increased in leaves
(p<0.001), in roots decreased (p<0.05). FQ decreased with 24h of
treatment (p<0.001), in roots. Conclusions: While in leaves an oxidative
stress was observed and GSH as a non enzymatic defense
parameter began to act, in roots the metabolic behavior is different.
With higher As concentration and 72 h of treatment an oxidative
stress is verified in soybean plants.