MATRIZ BIOENGENIERIZADA PARA REGENERACIÓN ÓSEA: EVALUACIÓN IN VITRO E IN VIVO

MARTIN,A; FELDMAN, S; MORENO GARCIA MF; MISSANA LM

Buenos Aires

Congreso; VIII Congreso de la Región Latinoamericana de IADR - LIV Reunión Científica Anual de SAIO; 2021

SAIO-IADR

The main of this work was to evaluate in vitro release of rh-PTH 1-34, from developed biomaterial. And in vivo study, about the regenerative capacity of critical sized defect (CSD) on rabbit calvariae. In vitro: The peptide was quantified by electro-chemiluminescence (Cobas 6000 Equipment, Roche Diagnostics), at various time intervals: 0, 4, 8, 24, 72, 96 and 120 hours; from 30 µg/4.5 mg at final concentration, pH 7.4. In vivo: 20 New Zealand rabbits, 6 months old, 3.5 kg ± 500 gr., received muco-periosteal flap and CSD (15 mm Ø). Animals were divided in control group (CG) without treatment, and experimental group (EG) with biomaterial. They were euthanized at 45 and 90 days. Diagnostic imaging was performed, by computed tomograph Cone Beam (CBCT). Histopathological studies were done on decalcified samples using oriented sections and staining with H&E, Trichrome Masson and AB-Pas. In addition, ground undecalcified sections (MMA) were evaluated by transmitted light, polarized light and fluorescence microscopy. Digital images obtained by Soft CellSens 1.16, were morphometrically analyzed by Image ProPlus software. Mann Whitney test (Minitab 17) was selected for statistical data analysis. The release of rh-PTH 1-34 from the biomaterial was confirmed until 96 h. Bone obtained from CBCT was at 45d and 90d CG: 20% and 32% respectively; while EG was 43% at 45 d and 73% at 90 d. The histomorphometric results were: CG at 45d and 90d, 16% and 22% of new bone formation, respectively; and EG obtained 45% at 45 d and 89% at 90 d. Significant differences were found between the selected groups (p