The aqueous extract of an argentinian medicinal plant (Smilax campestris) induce osteoblast differentiation, acting at oestrogen receptors (ER) alpha

MANGONE F; SOTELO A; SALAVERRY L; PARRADO CECILIA; ALMOZNI B; REY-ROLDÁN ESTELA; CASTRO MS; CANELLADA AM

Mar del Plata

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; 2018

Sociedad Argentina de Inmunología

Smilax campestris is a medicinal plant traditionally used as antirheumaticdue to its anti-inflammatory properties. We are interestedin to evaluate whether Smilax campestris have a bone-protectiverole during estrogen deprivation. Previously we demonstrated thataqueous extract of Smilax campestris (SM) inhibited osteoclast differentiationand induced osteoclast apoptosis, acting at ERα. Theaim of the present work was to evaluate whether SM could regulatethe osteoblast differentiation. To elucidate this, we analyzed in vitrothe effect of SM on the ability of MC3T3-E1 preosteoblastic cell lineto secrete mineral and organic matrix and to compare this effectwith the one of estradiol. Cells were cultured with or without SM(10, 100, 1000 ng/ml), estradiol (10-8, 10-7, 10-6 M), or with Ascorbicacid (50 μg/ml) and β-glycerophosphate (10 mM) as positive control,during 7 and 14 days. To analyze the role of ER, MC3T3-E1cells were incubated for 1h with ER antagonists previous to SM orestradiol treatment. The medium and stimuli were replaced every2 or 3 days. Then we analyzed in these cells the bone specific alkalinephosphatase activity (ALP), using the BCIP/NBT substratesystem, as well as calcium and collagen deposition by alizarin redand sirius red staining, respectively. Cells were lysed and stainingwas measured by spectrophotometry. Statistical analysis was appliedand differences were considered significant at a P