The Study of a polymeric adjuvant (Montanide Gel 01) in a vaccine against Bovine Respiratory Disease

ALMOZNI B; DÍAZ AILÉN MAGALÍ; MANGONE F; CANELLADA AM; CASTRO MS

CABA

Congreso; Reunión Conjunta de Sociedades Biomédicas; 2017

Sociedad Argentina de Inmunología

Bovine Respiratory Disease (BRD) is a multi-factorial disease that causes high morbidity and mortality in cattle. The disease involves a sequential cascade of events initiated by stress; thus, predisposing animals to viral infections which facilitate rapid bacterial invasion from the upper respiratory tract to lungs. Among bacteria causing BRD Mannheimia haemolytica (MH), Pasteurella multocida (PM) and Histophilus somni are the most important. Although BRD vaccines are worldwide produced and applied, there is a need of new therapeutic-adjuvant strategies in order to minimize the economic impact of respiratory syndrome.Montanide Gel 01 is a polymeric adjuvant, based on a dispersion of stable gel particles of sodium polyacrylate in water, which enhance the immune response by an antigen sustained release and activation of innate immune cells.The aim of this work was study the action Montanide Gel 01 (Seppic), when it is employed in the BALB/c mice immunization with MH and PM, both bacteria causing BRD. Mice were subcutaneously immunized on days 0, 15 and 27 with inactivated MH or PM formulated with Montanide Gel 01 at 5% or 10%, or with aluminum hydroxide (AH). Commercial vaccine was employed as positive control. Control mice received the adjuvants with PBS. Blood samples were obtained on days 0, 10, 27 and 38 and serum anti-PM and anti-MH IgG, IgG1, and IgG2a levels were measured by in house indirect ELISA.Montanide Gel 01 induced an increase in the anti-MH IgG, IgG1 and IgG2a levels compared with AH vaccine (p< 0.0001; p< 0.001; p< 0.01, respectively). In relation with anti-PM antibodies, IgG2a levels were higher than the commercial vaccine (p< 0.001). Specific IgG and IgG1 levels showed the same tendency although no significant differences were found. To conclude, Montanide Gel 01 induced a strong humoral immune response. Further studies in cattle will allow us analyze Montanide Gel 01 as a possible adjuvant candidate to use in BRD vaccines.