Study of the immune response elicited by a commercial vaccine against Bovine Respiratory Disease in a murine model

DÍAZ AILÉN; COGNARD ANAÏS; GONZÁLEZ MAGLIO D; CANELLADA ANDREA; MANGHI MA; CASTRO MS

Mar del Plata

Congreso; Reunión Anual de la Sociedad Argentina de Inmunología; 2014

Sociedad Argentina de Inmunología

Bovine respiratory Disease (BRD) constitutes one of the most common diseases that affect cattle during the first months of life. Pasteurella multocida (PM) and Mannheimia haemolytica (MH) are included among the complex etiologic agents responsible of BRD. Although vaccines against BRD are broadly employed in Argentine, they do not always provide an adequate control of the pneumonic form of the disease. To improve actual formulations, it is necessary understand the immune response elicited by these vaccines. The present work attempts to establish a murine model to study the immune response elicited by a BRD commercial vaccine. Female BALB/c mice were immunized with 0.25 ml of BRD vaccine by subcutaneous route at days 1 and 15. Twelve days after the last immunization mice were bled and sacrificed. Specific humoral and cellular immune response against PM and MH were measured. IgG levels in serum were measured by ELISA, spleen cell proliferation was determined by 3H thymidine uptake, and cytokine levels in spleen cell supernatants were analyzed by capture ELISA. Immunization elicited specific IgG levels against PM (p