A plant extract from Smilax campestris (SME) induces mitophagy and reduces arsenic-induced pro-inflammatory effect in human keratinocytes

SALAVERRY L; LOMBARDO T; PARRADO CECILIA; MANGONE F; MACCHI R; KORNBLIHTT L; RUGNA A; CASTRO MS; CANELLADA AM; REY-ROLDÁN ESTELA; BLANCO G

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

Smilax campestris is an Argentinian plant traditionally used to treatinflammatory skin disorders. Arsenic causes a skin disease knownas HACRE and mitochondrial damage with pro-inflammatory effects.Mitophagy (MF) ensures mitochondrial quality. We have previouslyshown that arsenic trioxide (ATO) increases IL-1 secretion inhuman keratinocytes (HaCaT) that can be reduced by drug-inducedMF. Here we explore if an aqueous extract of Smilax campestris(SME) induces MF and reverts ATO-induced IL-1 production in Ha-CaT cells. Mitochondrial mass (MM) was evaluated with the probeNAO by flow cytometry. MF was assessed with Hoescht, lysotracker,NAO, expression of Emerald Green-p62, and a mitochondria-targetedtandem EGFP-RFP protein (mtTan), by image cytometry. IL-1levels were measured by ELISA. We observed that SME (0.1-10μM)decreased MM. ATO-treated cells showed decreased IL-1 productionwhen co-treated with SME (p