Determination of multiple emerging pollutants in effluents from dairy production?

FRANCA GIANNINI KURINA; MIRTA NASSETTA; JULIETA BORELLO; IRENE CAÑAS; DIEGO GRASSI; VILLAAMIL LEPORI

virtual

Workshop; 8 LATIN AMERICAN PESTICIDE RESIDUE WORKSHOP; 2021

Latin American Pesticide Residue Workshop

Emerging pollutants (EPs) refer to compounds of different origin and chemicalnature, whose presence in the environment can generate alterations or adverse effectson biotic and abiotic systems. Its presence was not previously known as they wereunnoticed. These compounds reach the environment through domestic and industrialwastewater (treatment plants, hospital effluents, agricultural and livestock activities).The intensification of livestock activity can cause contamination of soils and water(surface, underground and drinking water) and can generate public health problems.The fate of substances used in animal production for health purposes has been littlestudied. Animal manure and effluents generated from livestock are commonly used inagriculture as fertilizers. To assess the health risk of the presence of these EPs(veterinary drugs), proper identification and quantification in various environmentalmatrices is necessary, which is why it is important to have analytical methodologies andequipment of high specificity and sensitivity such as coupled liquid chromatography-tandem mass spectrometry (LC-MS / MS).In the present work, multiple EPs were identified and characterized in effluent watersamples from dairy activity.Thirty samples of effluent waters were taken from dairy farms in the Northwest regionof the province of Córdoba during the period September-December 2020. The analysisof the effluent samples was carried out in an LC-MS / MS; the parameters of each wereoptimized. Compound on the mass spectrometer, the potentiality of the linear ion trapinstrument (QqLIT) employed was exploited, including enhanced scans such asenhanced product ion spectra (EPI), enhanced resolution (ER) and MS3experiments.Due to the wide variability of physical and chemical properties that these moleculespresent, the analytical procedure was based on a treatment of the effluent with organicsolvents, 0.1M disodium EDTA, 0.1M dibasic potassium phosphate buffer and pH 8.5,membrane filtration and subsequent quantitative determination by LC-MS / MS.Veterinary drugs investigated were antiparasitics, anti-inflammatories and antibioticsof the family of beta-lactams, quinolones, macrolides, tetracyclines, sulfonamides. Theconcentrations detected and quantified in the effluent were found within the range 0.1-20 μg.L-1, with recoveries for the different analytes between 50% and 130%.These initial results provide information for the development of knowledge for thegeneration of future regulations in the province of Córdoba on the use and disposal oflivestock waste.