Emerging drugs in environmental matrices of the province of Córdoba as a consequence of veterinary use in dairy production

BORELLO, JULIETA; MIRTA NASSETTA; IRENE CAÑAS; FRANCA GIANNINI KURINA; VILLAAMIL LEPORI

virtual

Encuentro; SETAC Latin America 14th Biennal Meeting; 2021

Society of Environmental Toxicology and Chemistry

In recent years there has been an increase in the use of veterinary drugs such asantibiotics, antiparasitic drugs, and others in livestock. Animal manure and effluentgenerated by livestock are often used in agriculture as fertilizer, a practice that couldrepresent a risk of contamination of agricultural production by these products. Theaccumulation of these residues in the soil degrades it and the presence of antibiotics cangenerate resistant microorganisms. Drugs and their metabolic products are dischargedinto the environment through waste (feces and urine excreted by animals). Waterwaysare an important transport route for used veterinary waste. The analysis of drug traces inenvironmental samples is known to present certain difficulties, such as the lowconcentration in these samples, matrix interferences during analysis, and the lack of afast, efficient and reliable extraction method. Therefore, the investigation of thesepollutants in different environmental matrices requires separation and identificationtechniques that are efficient, selective, and sensitive. The objective of this work was toevaluate the presence of veterinary drugs in environmental matrices from dairyproduction in the province of Córdoba.Fifty establishments were sampled during the period September-December 2020,January-May 2021. Samples of liquid effluent, manure, and agricultural soil were taken.The drugs investigated were: Antibiotics (Enrofloxacin, Amoxicillin, Ampicillin,Penicillin, Spiromycin, Ceftiofur, Tylosin, Tetracycline, Oxytetracycline),Antiparasitics (Ivermectin and Abamectin), and the Anti-inflammatory Flunixin. In thisstudy, the extraction methods of the analyzed drugs were developed and adapted and theconditions of the instrumental analysis by liquid chromatography coupled to tandemmass spectrometry were optimized. The parameters analyzed were satisfactory andfulfilled the bibliographic validation criteria: correlation coefficients for the calibrationcurves R2 = 0.990-0.9999, recovery range: 50-130%, relative standard deviation ofprecision: