Correlation between salivary and serum markers of bone turnover in osteopenic rats.

PELLEGRINI GRETEL G; GONZALES CHAVES MS; SOMOZA JULIA C; FRIEDMAN SILVIA M; ZENI SUSANA NOEMI

JOURNAL OF PERIODONTOLOGY

American Academy of Periodontology

Lugar: USA; Año: 2008 vol. 79 p. 158 - 165

0022-3492

Background: Previously, we measured bone alkaline phosphatase (b-ALP) and terminal C-telopeptide of collagen type I (CTX) in saliva. The present longitudinal experimental study sought to determine whether salivary concentrations of b-ALP and CTX have the same response as in serum samples under different conditions: normal, increased, and reduced bone remodeling. (b-ALP) and terminal C-telopeptide of collagen type I (CTX) in saliva. The present longitudinal experimental study sought to determine whether salivary concentrations of b-ALP and CTX have the same response as in serum samples under different conditions: normal, increased, and reduced bone remodeling. (b-ALP) and terminal C-telopeptide of collagen type I (CTX) in saliva. The present longitudinal experimental study sought to determine whether salivary concentrations of b-ALP and CTX have the same response as in serum samples under different conditions: normal, increased, and reduced bone remodeling. (b-ALP) and terminal C-telopeptide of collagen type I (CTX) in saliva. The present longitudinal experimental study sought to determine whether salivary concentrations of b-ALP and CTX have the same response as in serum samples under different conditions: normal, increased, and reduced bone remodeling. Previously, we measured bone alkaline phosphatase (b-ALP) and terminal C-telopeptide of collagen type I (CTX) in saliva. The present longitudinal experimental study sought to determine whether salivary concentrations of b-ALP and CTX have the same response as in serum samples under different conditions: normal, increased, and reduced bone remodeling. Methods: Thirty rats were ovariectomized (OVX) to induce osteopenia 60 days after surgery, and 10 rats were sham operated. Then, the ratswere divided into four groups and treated as follows for 45 days: group 1 (G1) = SHAM+ vehicle; group 2 (G2) = OVX + 8 mg olpadronate (OPD)/100 g of body weight; group 3 (G3) = OVX + 4 mg OPD/100 g of body weight; and group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 3 (G3) = OVX + 4 mg OPD/100 g of body weight; and group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 3 (G3) = OVX + 4 mg OPD/100 g of body weight; and group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 3 (G3) = OVX + 4 mg OPD/100 g of body weight; and group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (G2) = OVX + 8 mg olpadronate (OPD)/100 g of body weight; group 3 (G3) = OVX + 4 mg OPD/100 g of body weight; and group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. group 4 (G4) = OVX + vehicle. Saliva and serum CTX and b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. b-ALP were determined at 60 days (baseline) and at 75 days (T75). Lumbar spine and proximal tibia bone mineral density (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at baseline and at 105 days. (BMD) was determined using dual-energy x-ray absorptiometry at