ANTIGEN PRESENTING CELLS PULSED WITH INACTIVATED FMDV REALEASE EXTRACELLULAR VESICLES WITH THE ABILITY TO ACTIVATE BOTH SPECIFIC T AND B-CELLS IN VITRO.

FLORENCIA MENAY; ANALÍA ELISEI; ALEJANDRA FERELLA; COCOZZA, FEDERICO; JAVIER RE; CLAUDIA MONGINI.; GRAVISACO, MARÍA J; SAMPEDRO, PURA

CABA

Congreso; REUNION ANUAL DE SOCIEDADES DE BIOCIENCIAS; 2020

SAI-SAIC-SAFE

ANTIGEN PRESENTING CELLS PULSED WITH INACTIVATED FMDV REALEASE EXTRACELLULAR VESICLES WITH THE ABILITY TO ACTIVATE BOTH SPECIFIC T AND B-CELLS IN VITRO.Autores: Florencia Menay, Federico Cocozza, María José Gravisaco, Analía Elisei, Javier Re, Pura Sampedro, , Alejandra Ferella y Claudia Mongini.Foot-and-mouth disease (FMD) is a highly contagious disease of livestock worldwide and is economically important. The main strategy for the control is vaccination with FMD-Virus (FMDV) chemically inactivated with binary ethylenimide (FMDVi). In FMDV infection and in vaccination, the B cell response plays a major role by providing neutralizing/protective antibody in both animal models and natural hosts. Extracellular vesicles (EVs) are nanovesicles involved in cell?cell communication. EVs secreted by antigen-presenting cells (APC) participate in the activation of B and T cells through the presentation of native antigen membrane associated (to B cells) or by transferring MHC-peptide complexes (to T cells) and even complete antigen from DCs. In our previous work we demonstrated that murine APC cells can internalize FMDVi and release EVs expressing APC markers and high level of viral proteins during the first 24 h. In the present work we aimed to evaluate the immune properties of these EVs in the generation of B and T cell response against FMDV. We demonstrated that EVs-FMDVi induced specific in vitro proliferation in vivo sensitized splenocytes with FMDVi, EVs-FMDVi induced specific B cell (16.05% ± 0.61 p