Development and validation of a bovine parainfluenza virus type 3 indirect ELISA

MAIDANA, SS; PARREÑO, V; FERRECCIO, C; ROMERA, SA; ODEON, MM; GRAZZIOTTO, N

International Journal of Environment, Agriculture and Biotechnology

IJEAB

Año: 2021 vol. 6 p. 196 - 205

2456-1878

Serological assays, including enzyme-linked immunosorbent assays (ELISA), provide an usefultool for screening animals for the presence of antibodies (Abs) against a wide range of infectious agents(including viruses that cause respiratory disease in cattle) and are mainly used in veterinary medicine toassist to the control and disease?s monitoring. The aim of the present study was developing and validatingone indirect enzyme-linked immunosorbent assay (ELISA) based on semi purified bovine parainfluenzavirus type 3 (BPIV3).This test would allow to detect and quantify Abs against PI3 in serum sample fromcattle and guinea pigs on both purposes diagnostic and typify/specify the quality of vaccines. Thediagnostic sensitivity and specificity from the assay was 88% and 100% for bovine samples, using athreshold of corrected optical density, ODc =0.300, and 91% and 100% for guinea pig samples with aODc =0.250.The intermediate precision expressed as the assays positive control coefficient of variation(CV) was 20% for bovines and 8.5% for guinea pigs. Both techniques reproducibility obtained in interlaboratoryassays was CV=17% for bovines and 15% for guinea pigs, which found the requirements ofOIE (CV