Association of TNFα rs668920841 and INRA111 polymorphisms with caprine brucellosis: A case-control study of candidate genes involved in innate immunity

HASENAUER, FLAVIA C.; RASCHIA, MARIA A.; ROSSETTI, CARLOS A.; ROSSI, URSULA A.; MAURIZIO, ESTEFANIA; CAFFARO, MARIA E.; POLI, MARIO A.

GENOMICS

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2020 vol. 112 p. 3925 - 3932

0888-7543

In this study, we evaluated if polymorphisms at innate immunity-related genes were associated with susceptibility or resistance to Brucella infection in goats. Nine polymorphisms within IFNG, TNF, MYD88, IL10 and IL10RA genes and two molecular markers (BMS2753 and INRA111) were resolved by PCR-capillary electrophoresis in samples from 81 seronegative and 61 seropositive goats for brucellosis. A heterozygous genotype at INRA111, a microsatellite near the VRK serine/threonine kinase 2 (VRK2) gene, was associated with absence of Brucella-specific antibodies in goats naturally exposed to the pathogen (P = .004). Conversely, variants in the TNF gene (rs668920841) and near the IFN gamma receptor 1 (IFNGR1) gene (microsatellite BMS2753) were significantly associated with presence of Brucella-specific antibodies at allelic and genotypic level. Moreover, an in silico analysis predicted a functional role of the insertion-deletion polymorphism rs668920841 on the transcriptional regulation of the caprine TNF gene.