A rapid, non-destructive and highly efficient A. rhizogenes-mediated hairy root transformation protocol based on colour selection

MIRAS S. L; TIRAPU O.; CACACE J.; GABRIELA NATALIA CAMPOS; DIEGO LIJAVETZKY

Mendoza

Congreso; LVIII Reunión Anual de la Sociedad Argentina de Investigación en Bqca y Biología Molecular; 2022

Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology. On the other hand, reporters have been widely used to visualize gene expression, protein localization, and other cellular activities, but the commonly used reporters require special equipment, expensive chemicals, or invasive treatments. Here, we pursue to develop a rapid, non-destructive, and highly efficient method to analyze gene expression, based on a carrot (Daucus carota L.) Hairy Root (HR) system, induced by Rhizobium rhizogenes (A. rhizogenes).