Comparison of seven structurally related coumarins on the inhibition of Quorum sensing of Pseudomonas aeruginosa and Chromobacterium violaceum

D'ALMEIDA ROMINA ELISA; MOLINA ROCIO; VIOLA CAROLINA; LUCIARDI MARIA CONSTANZA; NIETO PEÑALVER CARLOS; BARDON ALICIA; ARENA MARIO E

BIOORGANIC CHEMISTRY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2017 vol. 73 p. 37 - 42

0045-2068

Quorum sensing (QS) is a cell-to-cell signaling communication system that controls the virulence behaviorof a broad spectrum of bacterial pathogens, participating also in the development of biofilms, responsibleof the antibiotic ineffectiveness in many infections. Therefore, QS system is an attractive target forantimicrobial therapy. In this study, we compare the effect of seven structurally related coumarinsagainst bacterial growth, biofilm formation and elastase activity of Pseudomonas aeruginosa. In addition,the anti-pathogenic capacity of the seven coumarins was evaluated on the wild type and the biosensorstrain of Chromobacterium violaceum.The comparative study of coumarins showed that molecules with hydroxyl groups on the aromatic ringdisplayed higher activity on the inhibition of biofilm formation of P. aeruginosa over coumarins with substituentsin positions 3 and 4 or without the double 3,4-bond. These 3 or 4-hydroxylated positions causeda decrease in the anti-biofilm activity obtained for coumarin. However, the hydroxyl group in position 3of the pyrone ring was important for the inhibition of C. violaceum QS and elastolytic activity of P. aeruginosa.The effects observed were active independently of any effect on growth. According to our results,coumarin and its hydroxylated derivatives represent an interesting group of compounds to use as antivirulenceagents against the human pathogen P. aeruginosa.