Chromatographically isolated exosomes from mesenchymal stem cells over-expressing heme oxygenase-1 for tissue regeneration

SANTA CRUZ, DM; PACIENZA, N; DONG-KI, K; MALVICINI, R; PROCKOP, D; YANNARELLI, GG

Congreso; LXI Reunion de la Sociedad Argentina de Investigaciones Clinicas; 2016

Regenerative properties of mesenchymal stem cells (MSCs)are largely mediated by paracrine mechanisms, including the secretion of extracellular vesicles (EVs). Exosomes are EVs ofendosomal origin that may constitute a safe and effective cell-freetherapy. We developed a scalable chromatographic procedure forproduction of exosomes derived from human MSCs over-expressingheme oxygenase-1 (HO-1) that might have anti-inflammatory/immunosuppressive potential. MSCs were activated with hemin,an HO-1 inducer, for 24 h and subsequently cultured in a chemicallydefined protein-free media (CDPF) during 48 h to collect theexosomes. Hemin treatment up-regulated HO-1 mRNA expressionby 54±14-fold compared with control MSCs (p