INVESTIGADORES
SANTA CRUZ Diego Mario
congresos y reuniones científicas
Título:
HO-1 involvement in the protection exerted by salicylic acid in Cd treatment in soybean plant
Autor/es:
NORIEGA, GO; CAGGIANAO E; LOPEZ LECUBE, M; SANTA CRUZ, DM; TOMARO ML.; BALESTRASSE, KB
Lugar:
Edinburgo
Reunión:
Congreso; 7th international meeting on heme oxygenases and related enzymes.; 2012
Resumen:
In higher plants, Cd promotes the accumulation of reactive oxygen
species, causes severe damage to important cellular components, such as
lipids, protein, DNA, and RNA, and leads to a decreased growth, roll and
chlorosis of leaf, and root necroses. Salicylic acid (SA) is an
endogenous growth regulator, which participates in physiological
processes in plants, such as growth, photosynthesis, nitrate metabolism,
ethylene production and flowering. SA is also known to be involved in
abiotic stress signaling, including plant response to heavy metals.
Aims. Experiments were carried out in order to elucidate the role of SA
pre-treatment on HO-1 behaviour in Cd treated soybean plants.Methods and
Results .Soybean plants were pretreated with different SA
concentrations for 48 h before 5 days treatment with 50 µM Cd. We
demonstrated that Cd brought about oxidative stress, measured as TBARS
formation, GSH and chlorophyll contents, as well as, antioxidant enzymes
such as , catalase, superoxide dismutase and guayacol peroxidase. Under
these conditions HO-1 activity and protein content were increased
(50%). Pre-treatment with SA prevented oxidative stress and provoked a
lower enhancement in HO-1 activity and protein content (30%). Atomic
absorption spectrometry indicated that Cd uptake is diminished in the
presence of SA. Conclusions. These findings highlight on one hand, that
SA inhibits Cd uptake and on the other hand enhances HO-1 activity and
protein content in soybean plants.