INVESTIGADORES
SANTA CRUZ Diego Mario
congresos y reuniones científicas
Título:
Heme oxygenase-1 and abscisic acid affect mapks gene expression in soybean seeds
Autor/es:
GIACOMETTI, R; SANTA CRUZ, DM; NORIEGA, GO; BALESTRASSE, KB
Lugar:
Mendoza
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).; 2012
Resumen:
In soybean previous studies enabled the identification of MAPK3
and 6 whose activity is enhanced within the signaling pathway
leading to defense reactions. In this study the effects of different
compounds related to hemeoxygenase (HO-1) biosynthesis on
mitogen-activated protein kinase (MAPKs) genes expression in
soybean seeds were tested. To this end, 20μM hemine, 22μM
ZnPPIX, 0.5mM furidine or 100μM 8-bromoguanosine 3',5'-cyclic
monophosphate (8Br) were added to pre-hydrated seeds for 5 days.
MAPKs genes expression was enhanced in seeds treated with
hemine. This result indicates that heme catabolism could be
involved in the signaling mediated by this cascade pathway. To
confirm this hypothesis experiments were carried out in the
presence of ZnPPIX, a potent irreversible HO-1 inhibitor. In this
case, no gene induction was observed. On the other hand, 8Br, a
cGMP analog, induced HO-1 gene expression but did not modulate
MAPKs, indicating that this effect could not be mediated by
cGMP. When the action of furidine, an abscisic acid inhibitor, was
tested a diminution of HO-1 gene expression was observed. In this
regard, MAPKs showed a different response, being MAPK6 the
only transcript that showed a diminished respect to controls, while
MAPK3 mRNA as well as MAPKK1 was enhanced. These resultswere confirmed by western blotting and activity determinations