Dendritic cell migration to regional lymph nodes contributes to Yersinia enterocolitica O:3-induced reactive arthritis in TNFRP55-/- mice

SILVA, JUAN EDUARDO; MAYORDOMO, ANDREA CONSTANZA; DAVE, MABEL NOEMI; AGUILERA MERLO CLAUDIA; ELIÇABE RICARDO JAVIER; DI GENARO MARÍA SILVIA

San Miguel de Tucuman

Congreso; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología; 2019

Background: Reactive arthritis (ReA) may develop after gastrointestinal or genitourinaryinfections. Dendritic cells (DCs) are central for T lymphocyte activation. We have reportedthat TNFRp55-/- mice develop chronic Yersinia enterocolitica (Ye)-induced ReA. On thearthritis onset (day 14), these mice presented an increase of migratory DCs(mDC:MHCIIhiCD11c+) and resident DCs (rDC:MCHIIintCD11c+) in mesenteric lymphnode (MLNs) and regional lymph nodes to joints (RLN). The role of DCs in ReA isunknown. The purpose was to study the contribution of DCs migration to ReA.Methods: C57BL/6 wild-type (WT) and TNFRp55-/- mice were orally infected with 1-5×108 Ye O:3. To block DC migration, fingolimod (FTY72) (5mg/kg) was administeredintraperitoneally at days 14, 17 and 20 after infection. Clinical score was calculated untilday 21, and DCs from RLNs were analyzed by flow cytometry. Bone marrow-derived DCs(BMDCs) of WT or TNFRp55-/- mice were in vitro infected with Ye O:3, and the CCR7expression was analyzed by flow cytometry. The IL12/23p40 and IFN-γ levels weremeasured by enzyme-linked immunosorbent assay (ELISA) in homogenates of the joints.Results: At day 21, FTY720-treated TNFRp55-/- mice decreased their arthritis clinicalscore and the absolute number of mDC and rDC (CD11b+ or CD11- subsets) in their RLNs(p