Effect of tumor necrosis factor receptor p55 deficiency on the response of peritoneal macrophages to Yersinia antigens

ELIÇABE J; DI GENARO S

Potrero de los Funes-San Luis-Argentina

Congreso; XXIV Reunión Científica de la Sociedad de Biología de Cuyo, IV Reunión Anual SAMIC; 2006

Sociedad de Biología de Cuyo-Sociedad Argentina de Microscopia

We demonstrated that TNFRp55 has a protective role in Yersinia enterocolitica O:3 reactive arthritis (ReA). The objective was to study the role of TNFRp55 on the macrophage response to Yersinia arthritogenic antigens. Thioglycollate elicited peritoneal macrophages from wild-type and TNFRp55-/- C57BL/6 mice were obtained. For killing assays, adherent cells were infected with Yersinia (moi 1:100) and intracellular bacteria were counted at 0 and 90 min. Lipopolysaccharide (LPS) and outer membrane proteins (OMP) were prepared. Macrophages were stimulated with LPS O:3, O:5, O:8 (10 µg/ml) or OMP O:3 (50 µg/ml). Nitric oxide (NO) production, as nitrite, was measured in 6, 48 and 72 h supernatants using Griess reagent. Morphological changes of the cells were evaluated. TNFRp55-/- macrophages showed impaired intracellular Yersinia killing (p