Role of IL-17A on autophagy during human tuberculosis

PELLEGRINI, J; TATEOSIAN, NANCY; AMIANO, N; MORELLI, MP; CASTELLO, FA; ROLANDELLI, A; LEVI, A; CASCO, N; PALMERO, DJ; COLOMBO MI; GARCIA, VE

Rosario

Congreso; Reunión Latinoamericana de Tuberculosis y otras micobacteriosis y XXIII Congreso Latinoamericano de Microbiología.; 2016

An appropriate immune response against Mycobacterium tuberculosis (Mtb) requires Th1 cytokines, but it has been shown that IFN- alone is not enough to the complete bacterial eradication. Actually, Th17 cells have been associated with Mtb infection. Autophagy is an immune effector mechanism against intracellular pathogens, positively modulated by Th1 cytokines, whereas Th2 cytokines show the opposite effect. Thus, the aim of this work was to study the role of IL-17A on autophagy during active human TB. This study has the approval of the Ethics Committee of the Hospital Dr. Muñiz. Monocytes (Mo) from TB and healthy donors (HD) were infected and cultured with Mtb-H37Rv± IL-17A (10ng/ml, 24h) and autophagy levels were analyzed by Flow cytometry and Immunofluorescence Microscopy against LC3-IIB. We observe that IL-17A increases autophagy against Mtb-H37Rv in Mo from HD and TB with strong immunity against Mtb (HR-TB) (p