Levels of circulating IgG antibodies against CFP10 and ESAT6 proteins differentiate active TB patients and recently infected individuals from latently infected subjects”

VITTI AGUSTIN; ZUAZO ROCIO; CASTELLO, ALEJANDRO; PERI IBAÑEZ, ESTEFANIA; MORELLI P; DONNOLI L; BAZAN ANA; ARMITANO R; STUPKA JUAN; GALLEGO C; PALMERO, DJ; TATEOSIAN, NANCY L.; GARCIA, VE; AMIANO, NICOLAS

Congreso; LXX Reunión Anual de la Sociedad Argentina de Inmunología (SAI),; 2022

INTRODUCTIONCONCLUSIONSAbstract ID: 626ABSTRACTMETHODSMycobacterium tuberculosis (Mtb), the agent responsible for the tuberculosis disease (TB) has beenhistorically one of the deadliest infectious human pathogens. Mtb is characterized by its capacity to enter intoa dormancy state that allows it to persist in an individual for years. This latent condition makes directdetection of bacterial components difficult and results in a significant obstacle for diagnosis. We havepreviously generated a fusion protein (Fp) of CFP10 and ESAT6 antigens that can be used for detecting Mtbinfected individuals in an IGRA test. We have also demonstrated that the antigen Rv2626c allows todiscriminate individuals with an established latent tuberculosis infection (LTBI) from tuberculosis patients(TBP), recently infected subjects (RI) and healthy controls (HC). Here, we analyzed levels of circulating IgGantibodies against Fp in these mentioned study groups. We used Fp to sensitize ELISA plates and measuredIgG specific antibodies in plasma samples. We first observed that levels of IgG antibodies against ESAT6and CFP10 could differentiate TBP and HC (O.D. TB = 0.67 ± 0.09; O.D. HC = 0.14 ± 0.03; ****p