M-TYROSINE EFFECT ON IMMUNOSUPPRESSION MURINE MODELS

DANIELA MONTAGNA; ARIEL STRAZZA; ALEJANDRA DUARTE; PAULA CHIARELLA; MARTIN ISTURIZ; RAÚL RUGGIERO; BÁRBARA REARTE

Ciudad de Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Meta-tyrosine (m-tyr) is an isomer of tyrosine with anti-metastaticeffects. This could be explained, in part, by the modulation ofthe inflammatory context in an immunosuppression (IS) state. Wehave studied the m-tyr effect on the immune response and the associatedinflammatory context in two IS murine models. In the firstone, BALB/c mice were immunosuppressed with increasing dosesof LPS and treated with m-tyr. The second model consisted in theadministration of m-tyr in advanced metastatic LMM3 tumor-bearingmice when IS was established and the subsequent challenge with asingle dose of LPS. Humoral immune response, after immunizationwith sheep erythrocytes, was evaluated through a hemagglutinationassay, citokines production by ELISA and splenic cell populationsby flow cytometry. M-tyr enabled a significant increase of the antibodies titre when it was administered simultaneously and at the endof the schedule of LPS-induced IS. [Mean ± SEM, IS=4,78±3,1%;IS-mtyr=68,25±34,6% p