Proteome variation of the rat liver after simple cold storage using an ex vivo model

ROSANO, GERMÁN L.; CAMILA KNECHT; CECILIA BALABÁN; JOAQUÍN RODRÍGUEZ; CECCARELLI, EDUARDO A.; EDGARDO GUIBERT

Santiago de Compostela

Congreso; XII EuPA Congress; 2018

European Proteomics Association

Background: Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that may affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. The aim of this study was to identify liver proteins potentially associated with injury during cold storage and/or normothermic reperfusion using the isolated preserved rat liver model. Methods: Livers from male rats were subjected to either (1) cold storage for 24 hs, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 hs followed by ex vivo normothermic reperfusion for 90 min. Then, livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by 2D gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine.Results: 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them had already been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury.Conclusion: Our results give new evidence in favor of proteins proposed as biomarkers for liver injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion.