Development of a peptide ELISA for the diagnosis of Equine Arteritis Virus

METZ GE; LORENZÓN EN; SERENA MS; CORVA SG; PANEI CJ; DIAZ S; CILLI EM; ECHEVERRÍA MG

JOURNAL OF VIROLOGICAL METHODS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2014 vol. 205 p. 3 - 6

0166-0934

A peptide-based indirect ELISA was developed to detect antibodies against Equinearteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of proteinM were designed, synthesized, purified and used as antigens either alone or combined.Ninety-two serum samples obtained from the 2010 equine viral arteritis outbreak, analyzedpreviously by virus neutralization, were evaluated by the ELISA here developed. The bestresolution was obtained using peptide GP5. The analysis of the inter- and intraplatevariability showed that the assay was robust. The results allow concluding that thispeptide-based ELISA is a good alternative to the OIE-prescribed 1 virus neutralization testbecause it can be standardized between laboratories, can serve as rapid screening, canimprove the speed of diagnosis of EAV-negative horses and can be particularly useful forroutine surveillance in large populations.