Association of BoLA-DRB 3.2 Alleles with Resistance and Susceptibility to Persistent Lymphocytosis in BLV Infected Cattle in Argentina

PANEI, C.J; SUZUKI, K; ECHEVERRÍA, MG; SERENA, M.S; METZ, G.E; GONZÁLEZ, E.T

International journal of dairy science

ACADEMIC JOURNALS INC

Año: 2009 vol. 4 p. 123 - 128

1811-9743

The objective of this preliminary study was to describe alleles of bovine major histocompatibility complex class II BoLA-DRB3.2 gene associated with resistance and susceptibility to Persistent Lymphocytosis (PL) in Bovine leukemia virus (BLV) infected cattle, to set a direction for the future studies. A total of 81 dairy cattle in Argentina were investigated for distribution of BoLA-DRB3.2 alleles. The study cattle were categorized into 3 groups as follows: Category BLV- (BLV seronegative). The alleles were identified by polymerase chain reaction with subsequent analysis of restriction fragment length polymorphism. The association between the BoLA-DRB3.2 alleles and the PL+ and PL- categories was determined as the odds ratio. Some alleles BoLA-DRB3.2 mediating (1) resistance and (2) susceptibility to PL were observed, respectively. A stochastic model including Shannon?s diversity index calculation was run based on Markov-Chain Monte Carlo simulation method. The Shannon?s diversity indices in each of the cattle categories indicated that the richness of gene diversity decrease as leading to a clinical stage of BLV. This relates to the existence of alleles mediating resistance and susceptibility to PL. The stochastic prediction with inclusion of uncertainty is important which can serve as a future study beacon.