INVESTIGADORES
ARIEL Federico Damian
congresos y reuniones científicas
Título:
Non coding transcription by alternative RNA polymerases dynamically regulates an auxin-driven chromatin loop
Autor/es:
ARIEL, FD; JEGU, T; LATRASSE, D; ROMERO-BARRIOS, N; CHRIST, A; BENHAMED, M; CRESPI, M
Lugar:
Poznan
Reunión:
Congreso; Post-Transcriptional Gene Expression Regulation in Plants; 2014
Institución organizadora:
Polish Society of Experimental Plant Biology
Resumen:
The eukaryotic epigenome is
shaped by the genome topology in three-dimensional space. Dynamic reversible
variations in the epigenome structure direct the transcriptional responses to
developmental cues. However, little is known about the control of epigenome
dynamics. Here, we show that the Arabidopsis
thaliana long intergenic non-coding RNA (lincRNA) APOLO is transcribed by RNA polymerase II (Pol II) and V (Pol V)
complexes in response to auxin, a phytohormone controlling numerous facets of
plant development. APOLO
transcription facilitates the formation of an oscillating chromatin loop
encompassing the promoter of its neighboring gene PID (or PINOID), a key
regulator of auxin polar transport. Chromatin and RNA Immuno-precipitation
(ChIP and RIP), together with Chromatin Isolation by RNA Purification (ChIRP)
and Chromatin Conformation Capture (3C) served to decipher the ncRNA-mediated
mechanisms controlling the chromatin loop opening and closing in response to
auxin, modulating PID promoter
activity. Components of the plant Polycomb Repressive Complexes as well as the
transcriptional gene silencing and DNA demethylation machineries contribute to
fine-tune chromatin loop dynamics. Altering APOLO
expression or its 24nt siRNA-dependent DNA methylation affects loop
formation and, consequently, PID
expression. Hence, the active transcription of a lincRNA by alternative RNA
polymerase complexes influences local chromatin topology and the expression of
a neighboring locus, leading to far-reaching consequences on a variety of
developmental outputs.