INVESTIGADORES
ARIEL Federico Damian
congresos y reuniones científicas
Título:
Non-coding RNAs in root developmental plasticity
Autor/es:
ARIEL, FD; ROMERO-BARRIOS, N; LATRASSE, D; BARDOU, F; BENHAMED, M; CRESPI, M
Lugar:
Jeju
Reunión:
Congreso; 10th International Congress on Plant Molecular Biology; 2012
Institución organizadora:
IPMB
Resumen:
Non protein
coding RNAs (npcRNAs) are emerging as key players in the regulation of varied
important cellular processes. Long npcRNA represent a class of riboregulators,
which either act directly in this long form or are processed into shorter small
si/miRNAs, leading to mRNA cleavage, translational repression or epigenetic
DNA/chromatin modification of their targets. In our Lab, a bioinformatic
approach served to identify 76 Arabidopsis
thaliana npcRNAs including several siRNA precursors and antisense RNAs.
Abiotic stress, such as phosphate starvation, drought or salt stress altered
the accumulation of many of these npcRNAs (1). The expression pattern of a set
of long npcRNAs in response to phytohormones and environmental stresses suggested
a link with root growth and development. Interestingly certain 24nt siRNAs derived
from these loci accumulate under the same conditions, suggesting an epigenetic
link.
The overexpression
of one auxin-responsive long npcRNA, npc34, in insertion promoter mutants and
transgenic lines, leads to an acceleration of root growth and an altered
response to gravity. Transcript levels of npc34 correlate with the expression
of its neighbor gene PINOID (PID), a primary auxin-responsive gene
involved in the phosphorylation of auxin efflux regulators. Furthermore, ChIP-qPCR
experiments suggest that the npc34 over-accumulating lines have a modified
chromatin status, exhibiting several modifications at the histone level, as
well as in the spatial conformation of the chromatin. Our results suggest a
direct implication of npc34 in PID
expression regulation by chromatin remodeling, thus in the auxin control of root
development.
(1) B. Ben Amor, et al.: Novel long non-protein
coding RNAs involved in Arabidopsis differentiation and stress responses,
Genome Res. (2009) 19, 57-69.