Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature

PACHECO, JAVIER MARTÍNEZ; RANOCHA, PHILIPPE; KASULIN, LUCIANA; FUSARI, CORINA M.; SERVI, LUCAS; APTEKMANN, ARIEL. A.; GABARAIN, VICTORIA BERDION; PERALTA, JUAN MANUEL; BORASSI, CECILIA; MARZOL, ELIANA; RODRÍGUEZ-GARCIA, DIANA ROSA; DEL CARMEN RONDÓN GUERRERO, YOSSMAYER; SARDOY, MARIANA CARIGNANI; FERRERO, LUCÍA; BOTTO, JAVIER F.; MENESES, CLAUDIO; ARIEL, FEDERICO; NADRA, ALEJANDRO D.; PETRILLO, EZEQUIEL; DUNAND, CHRISTOPHE; ESTEVEZ, JOSÉ M.

NATURE COMMUNICATIONS

Nature Research

Año: 2022 vol. 13

2041-1723

Root Hairs (RHs) growth is influenced by endogenous and by external environmental signals that coordinately regulate its final cell size. We have recently determined that RH growth was unexpectedly boosted when Arabidopsis thaliana seedlings are cultivated at low temperatures. It was proposed that RH growth plasticity in response to low temperature was linked to a reduced nutrient availability in the media. Here, we explore the molecular basis of this RH growth response by using a Genome Wide Association Study (GWAS) approach using Arabidopsis thaliana natural accessions. We identify the poorly characterized PEROXIDASE 62 (PRX62) and a related protein PRX69 as key proteins under moderate low temperature stress. Strikingly, a cell wall protein extensin (EXT) reporter reveals the effect of peroxidase activity on EXT cell wall association at 10 °C in the RH apical zone. Collectively, our results indicate that PRX62, and to a lesser extent PRX69, are key apoplastic PRXs that modulate ROS-homeostasis and cell wall EXT-insolubilization linked to RH elongation at low temperature.