Correlation between PMCA activity and tubulin on platelet function in spontaneously hypertensive rats

ZANOTTO S; MUHLBERGER T; BALACH M; CAMPETELLI AN; CASALE C H; MONESTEROLO N E

Córdoba

Congreso; Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016

SAIB

In previous work we have shown that tubulin and PMCA form a complex, and the formation of this complex regulates the PMCA activity in normotensive and hypertensive subjects. It has previously been reported that increased calcium intraplatelet can cause hyperactivation of platelets during hypertension. Because of the importance of calcium regulation in platelets we decided to study the regulation of PMCA by tubulin in hypertensive rats. We observed a significant increase in acetylated and detyrosinated tubulin and presence of complex of tubulin and PMCA, suggesting the interaction both proteins. Analysis the PMCA activity revealed that the activity in platelets hypertensive rats is decreased by 50% compared to the control, and this decrease in activity correlates with a higher degree of phosphorylation on tyrosine residues in PMCA Functional analysis of platelets obtained by measurement of bleeding time indicates that in hypertensive rats the time to form platelet plug is 3 times less than that needed in Wistar rats. In summary would indicate a correlation between the high tubulin content, low PMCA activity and higher activation in platelets hypertensive rats.