Effects of detyrosinated tubulin on Na(+),K(+)-ATPase activity and erythrocyte function in hypertensive subjects.

AMAIDEN M R; SANTANDER VS; MONESTEROLO N E; NIGRA A; RIVELLI J F; CAMPETELLI A N; PIE JUSTE, J; CASALE C H

FEBS LETTERS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 589 p. 364 - 373

0014-5793

Formation of tubulin/Na+ ,K+ -ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. Moreover, we report a reduced content and activity of the enzyme tubulin tyrosine ligase in erythrocytes of hypertensive subjects. Such alterations are related to changes in erythrocyte deformability. Our findings indicate that the detyrosination/tyrosination cycle of tubulin is important in regulation of NKA activity, and that abnormalities in this cycle are involved in hypertension development.