Larrea divaricata’s fractions enhance the phagocytosis of Candida albicans by murine macrophages.

MARTINO R,; DAVICINO RC,; CASALI YA, .; MATTAR MA, ; MICALIZZI B

Ciudad de la Punta, San Luis

Congreso; XXVII Reunión Anual Científica de la Sociedad de Biología de Cuyo; 2009

Sociedad de Biología de Cuyo

Larrea divaricata is known for its multiple applications in folk medicine, It is known that decoction possess fungicidal effect. The aim of this work was to evaluate in vivo the effects on macrophages (MØ) treated with 3 fractions (F1, F2 and F3) and co-cultured with C. albicans. Mice were injected (i.p.) twice in a 48 hs period with 15 mg/kg of each fraction. Then, MØ were co-cultured with yeast. The following determinations were carried out: 1) phagocytosis by: a) Giemsa staining and b) NBT reduction; 2) NO production and 3) candidacidal assay. Results shows that F1 increase phagocytosis, NBT reduction (p<0.01) and NO production (p<0.05), but F1 doesn’t reduce the viability. On the other hand, F2 induce the increase of phagocitosis (p<0.05), but this increase was not observed on NO production and NBT reduction, nevertheless F2 shown a decrease of C. albicans viability (p<0.05). F3 increase the phagocytosis and NO production (p<0.05), but not the NBT reduction. In conclusion, while F1 exhibited microbicidal effects e.g. increase of NO and reduction of NBT, these are not enough to reduce the yeast viability in the experimental conditions. By contrast F2 does not present the same effects and reduces the viability of yeast as well as F3. This difference in the activity of fractions could be due to the relatively high content of nordihydroguaiaretic acid presenting F2 and F3 fractions and/or by the presence of others fungicidal compounds.is known for its multiple applications in folk medicine, It is known that decoction possess fungicidal effect. The aim of this work was to evaluate in vivo the effects on macrophages (MØ) treated with 3 fractions (F1, F2 and F3) and co-cultured with C. albicans. Mice were injected (i.p.) twice in a 48 hs period with 15 mg/kg of each fraction. Then, MØ were co-cultured with yeast. The following determinations were carried out: 1) phagocytosis by: a) Giemsa staining and b) NBT reduction; 2) NO production and 3) candidacidal assay. Results shows that F1 increase phagocytosis, NBT reduction (p<0.01) and NO production (p<0.05), but F1 doesn’t reduce the viability. On the other hand, F2 induce the increase of phagocitosis (p<0.05), but this increase was not observed on NO production and NBT reduction, nevertheless F2 shown a decrease of C. albicans viability (p<0.05). F3 increase the phagocytosis and NO production (p<0.05), but not the NBT reduction. In conclusion, while F1 exhibited microbicidal effects e.g. increase of NO and reduction of NBT, these are not enough to reduce the yeast viability in the experimental conditions. By contrast F2 does not present the same effects and reduces the viability of yeast as well as F3. This difference in the activity of fractions could be due to the relatively high content of nordihydroguaiaretic acid presenting F2 and F3 fractions and/or by the presence of others fungicidal compounds.in vivo the effects on macrophages (MØ) treated with 3 fractions (F1, F2 and F3) and co-cultured with C. albicans. Mice were injected (i.p.) twice in a 48 hs period with 15 mg/kg of each fraction. Then, MØ were co-cultured with yeast. The following determinations were carried out: 1) phagocytosis by: a) Giemsa staining and b) NBT reduction; 2) NO production and 3) candidacidal assay. Results shows that F1 increase phagocytosis, NBT reduction (p<0.01) and NO production (p<0.05), but F1 doesn’t reduce the viability. On the other hand, F2 induce the increase of phagocitosis (p<0.05), but this increase was not observed on NO production and NBT reduction, nevertheless F2 shown a decrease of C. albicans viability (p<0.05). F3 increase the phagocytosis and NO production (p<0.05), but not the NBT reduction. In conclusion, while F1 exhibited microbicidal effects e.g. increase of NO and reduction of NBT, these are not enough to reduce the yeast viability in the experimental conditions. By contrast F2 does not present the same effects and reduces the viability of yeast as well as F3. This difference in the activity of fractions could be due to the relatively high content of nordihydroguaiaretic acid presenting F2 and F3 fractions and/or by the presence of others fungicidal compounds.C. albicans. Mice were injected (i.p.) twice in a 48 hs period with 15 mg/kg of each fraction. Then, MØ were co-cultured with yeast. The following determinations were carried out: 1) phagocytosis by: a) Giemsa staining and b) NBT reduction; 2) NO production and 3) candidacidal assay. Results shows that F1 increase phagocytosis, NBT reduction (p<0.01) and NO production (p<0.05), but F1 doesn’t reduce the viability. On the other hand, F2 induce the increase of phagocitosis (p<0.05), but this increase was not observed on NO production and NBT reduction, nevertheless F2 shown a decrease of C. albicans viability (p<0.05). F3 increase the phagocytosis and NO production (p<0.05), but not the NBT reduction. In conclusion, while F1 exhibited microbicidal effects e.g. increase of NO and reduction of NBT, these are not enough to reduce the yeast viability in the experimental conditions. By contrast F2 does not present the same effects and reduces the viability of yeast as well as F3. This difference in the activity of fractions could be due to the relatively high content of nordihydroguaiaretic acid presenting F2 and F3 fractions and/or by the presence of others fungicidal compounds.C. albicans viability (p<0.05). F3 increase the phagocytosis and NO production (p<0.05), but not the NBT reduction. In conclusion, while F1 exhibited microbicidal effects e.g. increase of NO and reduction of NBT, these are not enough to reduce the yeast viability in the experimental conditions. By contrast F2 does not present the same effects and reduces the viability of yeast as well as F3. This difference in the activity of fractions could be due to the relatively high content of nordihydroguaiaretic acid presenting F2 and F3 fractions and/or by the presence of others fungicidal compounds.