Immunomodulatory activity of Larrea divaricata Cav (jarilla) I : Activation of mouse peritoneal macrophages.

DAVICINO R C; MATTAR M A; CASALI Y A; CORREA S; MICALIZZI B

Córdoba (Argentina)

Congreso; VII Congreso Latinoamericano de Inmunología.; 2005

Asociación Latinoamericana de Inmunología

The aim of this work was to evaluate the activation of macrophages by two extracts of jarilla. The infusion (I) and decocción (D) were obtained with 5 g of dried and powered material according to Argentinean Pharmacopoeia. Peritoneal macrophages from mice sensitized with proteosa-peptona were collected for: respiratory burst (NBT); phagocytosis of Candid albicans; expression of LPS receptors; production of NO and iNOS expression (Western Blot). Jarilla’s extracts at 0.2 mg/ml activated macrophages. Reduction of NBT (p £ 0.02) as well as phagocytosis of C. albicans (P £ 0.04) were increased only when macrophages were treated with D and zimosan. Macrophages treated with I and LPS showed a significant increase in the production of NO (p £ 0.02) and the iNOS expression was 6.8 fold higher as compared with untreated macrophages. The binding of LPS-FITC respect to the controls was increased in macrophages treated with D and I. We can conclude that both jarilla’s extracts at 0.2 mg/ml activate macrophages and increased NO production and iNOS expression. In addition, D increases the expression of zimosan and LPS receptors, while infusion only increases LPS receptors .