Evaluation of concentration efficiency of the Pseudomonas aeruginosa phage PP7 in various water matrixes by different methods

HUGO RAMIRO POMA; VERÓNICA BEATRIZ RAJAL; MARÍA DOLORES BLANCO FERNÁNDEZ; BARRIL PA; GIORDANO MO; MASACHESSI G; MARTINEZ LC; ISA MB; MARÍA CECILIA FREIRE; GABRIELA RIVIELLO LÓPEZ; DANIEL CISTERNA; NATES SV; VIVIANA ANDREA MBAYED

ENVIRONMENTAL MONITORING AND ASSESSMENT

SPRINGER

Lugar: Berlin; Año: 2012 p. 1 - 10

0167-6369

Enteric viruses monitoring in surface waters requires the concentration of viruses before detection assays. The aim of this study was to evaluate different methods in terms of recovery efficiencies of bacteriophage PP7 of Pseudomonas aeruginosa, measured by real-time PCR, using it as a viral control process in water analysis. Different nucleic acid extraction methods (silicaguanidiniumthiocyanate, a commercial kit (Qiagen Viral RNA kit) and phenol-chloroform with alcohol precipitation) exhibited very low recovery efficiencies (0.08 to 4.18 %), being the most efficient the commercial kit used for subsequent experiments. To evaluate the efficiency of three concentration methods, PBS (as model for clean water) as well as water samples from rivers were seeded to reach high (HC, 106 pfu/mL) and low concentration (LC, 104 pfu/mL) of PP7. Tangential ultrafiltration proved to be more efficient (50.3612.91, 17.219.22, and 12.582.35 % for HC in PBS and two river samples, respectively)