Stillman Salgado rat (e-SS) a murine model to understand diabetes (DBT) development and brain complications

DIAZ-GEREVINI GT; LÓPEZ C; DAIN A; DIAZ C; DAS UN; EYNARD AR; REPOSSI G

Rio de Janeiro

Congreso; II Latin America Congress on Controversies to Consensus in Diabetes, Obesity and Hypertension; 2012

STILLMAN SALGADO RAT (E-SS) A MURINE MODEL TO UNDERSTAND DIABETES (DBT) DEVELOPMENT AND BRAIN COMPLICATIONS G. Díaz, C.Lopez, A. Dain, C. Díaz, D. Undurti, A. Eynard, G. Repossi Investigación, Instituto de Biología Celular, Argentina; Laboratorio, Sanatorio Punilla, Argentina; Investigación, Technological University, Kakinada, India Objective. Study in a murine model biological parameters associated with DBT development and complications. Materials and Methods: We used 20 rats e-SS (10 males and 10 females) developed at Rosario University, Argentina by selection and inbreeding and 20 Wistar (10 males and 10 females) as control. E-SS rat develop a mild spontaneous DBT 2. Glucose studies were performed with Accuchek Performa reagent, lipids and gamma glutamil transpeptidasa (GGTP) by Weiner Lab kit,histological techniques were hematoxylin-eosin, Nissl, and TUNEL technique for apoptosis (Apotag lab).Inmunohistochemistry anti Ac gene protein 9.5. The study of behavior was performed by Hole test (box of 50 x 50 cm with 4 holes in the base off the floor to 2 cm).Studied during 10 minutes, observed mobility, head deeping and raring. Euthanasia was performed using isoflurane. Results Male e-SS rat , develop hypertriglyceridemia after 4 months ,diabetes, associated with pancreatic apoptosis and elevated GGT after 6 months. Behavioural and brain histological changes (pyramidal cells loss in CA1 hippocampus area, gliosis and apoptosis) after 12 months. Female and Wistar rats remained normal.Conclusion This animal model is useful to understand DBT physiopathology.