Role of Ceramide Synthesis in Glial Activation and Communication After a Lipotoxic Stimulus

BELLOTTO M; BENTIVEGNA M; GREGOSA A; POMILIO C; GONZALEZ PEREZ N; PRESA J; SARAVIA F; BEAUQUIS J; VINUESA A

Buenos Aires

Congreso; FIRST MEETING GLIA CLUB SOUTHERN CONE; 2022

Saturated fatty acids (SFAs) are basic components of western-style diets. High systemic and cerebral levels promote chronic inflammation and metabolic disorders. This scenario could lead to brain dysfunction through multiple pathways and be a risk factor for neurodegenerative diseases. Previously, we have shown that a high-fat diet (HFD) induces central and peripheral inflammation, cognitive impairment, and hippocampal glial activation, in young and adult mice (Vinuesa et al., 2016, 2019). One of the mediators involved in these pathways are ceramides, lipidic molecules that, besides their physiological roles, could induce inflammation. In the present work, we aimed to study (1) the impact of palmitate (PA), one of the most abundant SFA in western-style diets, on glial activation and communication and (2) the role of ceramide synthesis mediating the effects of PA. Incubation of microglia (BV2 mouse cell line) with 0.5 μM PA-induced NFkB p65 nuclear translocation (p