LIPOTOXICITY- INDUCED METABOLIC INFLAMMATION: POTENTIAL ROLE OF CERAMIDES IN GLIAL CELLS INTERACTION AND INFLAMMATORY-DAMAGE PROPAGATION.

BELLOTTO M; VINUESA A; BENTIVEGNA M; GREGOSA A; POMILIO C; GONZALEZ PEREZ N; PRESA J; BEAUQUIS J; SARAVIA F

Congreso; Reunión de Sociedades de Biociencias 2021; 2021

Sociedad Argentina de Investigación Clínica (SAIC)

Western diet is associated with the elevated rates of obesity and related metabolic disorders,also considered important risk factors for brain dysfunction. Chronic inflammation and insulinresistance comprise the most relevant shared pathways, promoting alterations in theplasticity of limbic structures such as the hippocampus. With the aim to study the impact ofmetabolic disturbances on the brain we focused on the role of glial cells in two differentapproaches: in vivo, in the hippocampus of C57BL/6 mice exposed to a high fat diet (HFD)and in vitro, emulating the lipotoxic context with the saturated fatty acid palmitate (PA) as aninsult to microglia and astrocyte cell lines and the role of ceramide pathway.We have previously found that HFD mice exhibited decreased neurogenesis and structuralsynaptic alterations, together with spatial memory impairment and neuroinflammation, withincreased expression of hippocampal TNFα and IL1β cytokines and enlarged microglia.Here, we show that astrocytes also respond to the inflammatory status with a trend toincreased S100b staining and a higher cell complexity (assessed by GFAP labeling andSholl analysis RM-ANOVA p