Lung cancer and keratino cyte cells differential response to rural air particulate matter in vitro

NADIA ORONA; FRANCSICO ASTORT; IGNACIO FENOY; IMANOL PALAVECINO; RAMIREZ HABERKON NANCY; MENDEZ MARIANO; PANEBIANCO JUAN E; GOLDMAN ALEJANDRA

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

Atopic disorders susceptibility depends on interactions between genetic and environmental factors. The prevalence of these disorders has increased in the last 5 decades indicating that environmental factors are responsible for this increase. Airborne particulate matter (PM) is one of environmental health problems affecting the world´s population. Although several studies related the exposure to urban PM with the onset or exacerbation of allergies such as asthma and atopic dermatitis, there are few studies that link rural PM with the development of these allergic disorders. Rural PM comes mainly from agricultural activity, where agrochemical compounds are frequently used. Although agrochemical exposure has been related to allergic diseases, an approach that considers the synergistic effects of the interaction of PM and agrochemicals is necessary. Therefore, the aim of this study was to evaluate the effect of Rural PM from Pampeana region on pulmonary (A549) and skin (HaCaT) cells. A549 or HaCaT cells were exposed to soil generated-PM10 (1-100µg/ml) from an intensive cultivation area where the use of agrochemicals prevails, or glyphosate (0.75–7500pg/ml). After 24h, cell metabolic activity, lactate dehydrogenase (LDH) and cytokine release (IL-8, TSLP, IL-1β) was assessed. We found that A549 cells exposed to PM showed a marked increase in IL-8 production (P