INVESTIGADORES
ARRUVITO Maria Lourdes
congresos y reuniones científicas
Título:
A physiological role for Inducible FOXP3+ Treg cells. Lessons from Women with Reproductive Failure
Autor/es:
ARRUVITO LOURDES
Reunión:
Congreso; 1st Franco-Argentine Immunlogy Congress; 2010
Resumen:
A physiological role for Inducible
FOXP3+ Treg cells. Lessons from Women with Reproductive Failure
Lourdes Arruvito,* Ana I Sotelo, Ariel
Billordo,* and Leonardo Fainboim*
*Immunogenetics
Laboratory, José de San Martín Clinical
Hospital, School
of Medicine, University of Buenos Aires.
Department of Biochemistry, School of Biochemistry, University of
Buenos Aires, National Council for Scientific and Technological Research,
Buenos Aires, Argentina
We have previously shown a decreased frequency and function of Tregs in
women suffering from recurrent spontaneous abortions (RSA). In the current
study we first investigated the expression of FOXP3 after T cell activation. We
observed that expression of FOXP3 in activated PBMCs was already present above baseline
before any cell division, indicating that was induced in cells that were
previously negative for this transcription factor. Because RSA women showed a
more limited expansion of FOXP3 positive cells, we next assessed the role of
IL-2 signaling through STAT-5, which is known to be required for generation of
inducible Tregs (iTregs). We demonstrated not only that TGF-β and IL-2 were diminished but also that the
IL-2-STAT-5 signaling axis was down regulated in RSA women. In addition to a
limited FOXP3+ cells expansion in vitro, iTregs from RSA women showed a
strikingly lower suppressor activity. Finally, those
conditions in which iTregs may play a central role can be associated with the
expansion of Tregs observed in the pre-implantation phase of the menstrual
cycle and during pregnancy.