ONCOGENOMICS AND BIOINFORMATICS CORE ANALYSIS OF VIRALLY INDUCED AIDS-ASSOCIATED CANCERS

EZEQUIEL LACUNZA; WILLIAMS S; MESRI E; ABBA MC

Encuentro; Webex meeting: HIV-Associated Malignancy Research Centers-June 4th Call; 2021

I wil talk about the workflow of the OGBC:Samples derived from saliva, blood, oral and anal swabs from HIV infected and non-infected patients are collected by qualified staff at Fundación Huésped. Samples derived from ASCC are provided by the Hospital Udaondo. All samples are shipped to the OGBC where DNA and RNA are purified and stored. Selected DNA and RNA samples are then shipped to the UM for NGS and Atila HPV genotyping. DNA samples are also shipped to the Instituto Malbran for KSHV and HPV genotyping. Data eventually converge at the OGB Core for Bioinformatic Analysis.So far we have processed a total of one thousand two hundred forty-six (1246) samples.I will focus now on samples derived for microbiome analysis. This study is part of an ongoing project. Some results were presented at the MWS two thousand twenty (2020) The aim is to provide the first microbiome characterization of oral and anal samples derived from an Argentinian cohort of HIV infected and non-infected cases of TGW and MSM. A total of 80 samples of DNA derived from 40 Oral Swabs & 40 Anal Swabs were provided by 45 participants, of which 36 are MSM and 9 are TGW. The main differences observed in the clinical data of both groups were related with sexual behavior. In the age of anal sex initiation as well as in the number of sexual partners, considering that TGW included in this study are all sexual workers. We first analyzed all samples in an integrated way. Alpha diversity showed a significant difference among oral and anal samples in both virus and bacteria taxa.beta diversity shows two main clusters based on sample origin, which determine two locations completely different regarding microbial composition. Although a greater number of virus and bacterial species were identified in the anal samples, the oral samples showed a greater richness in the distribution of the abundance of species A few dominant and mutually exclusive taxa were identified. Species of Alphapapillomavirus were prevalent in anal samples while Human gammaherpesvirus 8 and 4, among others, were dominant in oral samples. In Bacteria, species of Prevotella were the most abundant in anal samples, while species of Rothia and Lautropia were prevalent in oral samples. For differential abundance analysis we made 12 comparisons between HIV- and HIV+ for virus and bacteria. Considering TGW and MSM together (green), and stratifying for sexual orientation: TGW (yellow) and MSM (blue). As we can see, in Oral Swabs KSHV was predominantly abundant in HIV+ patients independently of sexual orientation. Anal swabs of HIV+ showed enrichment mainly in the abundance of HPV types. For bacteria, there were significant variations in the abundance of species of Prevotella, and Veillonella among others. Next step is to increase the number of samples to 130 which is in progress now. We are going to investigate not only the microbial composition of the samples but also what metabolic processes these communities are involved in.